The Kruskal–Wallis and also the several Wilcoxon sum rank tests were being used. The level of importance was established at 0.05. Effects. The end result showed a nonsignificant difference between different irrigants from E. faecalis. Among the S. aureus subgroups, 2% CHX was statistically important and more effective than MTAD. https://www.directivepublications.org/journal-of-dentistry-and-oral-research/