This combination is then incubated at fifty five°C for one hour. Then the sample is phenol-extracted once with phenol/chloroform/isoamyl Liquor Answer and centrifuged. The aqueous layer is taken out to your contemporary microcentrifuge tube. The DNA is ethanol-precipitated, re-suspended in buffer, and ethanol-precipitated a 2nd time. Following the pellet is https://www.facebook.com/NewTechFind/posts/pfbid0d8VStcYignkfVz6MCZSKLaerVPpxVNKDb8CThGkvBTun4BMDH6o6PiDFY8YUrpQVl